Abstract: Flame atomic absorption spectrometry was used to determine gold in low-grade gold ore. The optimal measurement conditions were explored from 4 aspects： processing particle size， roasting conditions， activated carbon adsorption recovery rate， and atomic absorption spectrophotometer verification during experiments. The sources of relevant errors in the analysis process of low-grade gold ore were analyzed， and the experimental testing method and process were further optimized to improve the stability of the testing data. The method has high accuracy and precision， with a relative standard deviation (n＝10) of less than 1 %， and is simple， practical， and suitable for the measurement requirements of mining production technical indicators.